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P11008
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR & Calcium Channel Targets
Presenter Zhenjun Diwu, ABD Bioquest, Inc., USA
Additional Authors: Jinfang Liao, Jianjun He, Desuo Yang

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCRs) and calcium channels. Fluo-8 NW has been developed to provide homogeneous fluorescence-based assays for detecting intracellular calcium mobilization across a broad spectrum of biological targets. The characteristics of its long wavelength, high sensitivity, and >200 times fluorescence enhancement upon calcium binding make Fluo-8 NW an ideal indicator for measuring intracellular calcium. In comparison with Fluo-4 NW, FLUO-3 AM and FLUO-4 AM, Screen Quest™ Fluo-8 NW gives the brightest signal, more than 2 fold brighter than Fluo-4 AM, and 4 times brighter than Fluo-3 AM. It also has larger assay window than Fluo-3 AM and Fluo-4 AM-based calcium assays. Unlike Fluo-3 AM and Flu-4 AM, both of which require 37 °C for optimal cell loading, Fluo-8 NW has a less temperature-dependent cell loading property, giving consistent results either at room temperature or 37 °C. This characteristic makes Fluo-8 NW much more robust for HTS applications.