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P12001
An Ion Channel FLIPR HTS Using the New Fluo-8 Calcium Dye
Presenter Qing-Fen Gan, Roche Palo Alto LLC, USA
Additional Authors: Hoa Truong and Bruce Koch

Ca2+ ion is an important secondary messenger involved in many physiological and signal transduction processes within a cell. A Ca2+ signal can be generated by both intracellular store release and extracellular entry. Intracellular Ca2+ concentration changes are commonly monitored using calcium-sensitive dye based FLIPR assay. Recently, a new Ca2+-dye, Fluo-8, was developed by ABDBioquest. Due to its super brightness and low cost, we evaluated the Fluo-8 dye, developed a robust FLIPR HTS assay and completed an ion channel antagonist screening. A mean Z’-factor of 0.81 was achieved for the whole screening of >2200 plates. Comparative data between Fluo-8 and other Calcium assay kits, along with the HTS results will be presented.