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P2005
New SA-PE Conjugates Improve Critical Assay Parameters in Sandwich Immunoassays Performed Using Luminex® xMAP® Technology
Presenter Jo Wegstein, ProZyme, Inc., USA
Additional Authors: Bruce Amsden, Archie Chen, and Kerry Oliver
In Luminex® xMAP® assay development, the conjugated reporter dye streptavidin-phycoerythrin (SA-PE) can greatly influence such critical assay parameters as the signal strength, limit of detection, background and reproducibility of an assay. We present as an example a pair of antibody-coupled microspheres, which showed significant differences in these parameters. Several different types of SA-PE were evaluated and compared.