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P2027
Cellular Protein Dynamics Studied With Covalently Labeling Protein Tags
Presenter Andreas Brecht, Covalys Biosciences AG, Switzerland
Additional Authors: Andreas Brecht, Kathrin Muentener, Florent Beaufils, Olivier Roumanie
Protein dynamics is a key feature of living cells. Changes in protein expression and protein degradation are of high interest, but notoriously difficult to study. Also the transport of proteins during maturation or in response to external stimuli (e.g. receptor internalization & recycling) is of high relevance, but difficult to address. The Covalys protein tags (SNAP-tag, ACP-tag) are smaller than GFP and can be rapidly and irreversibly labeled with a substrate of choice. The range of substrates available is wide and includes fluorescent labels from the blue to the green and red. Already the simple labeling of proteins without any licensing requirements is a significant improvement. In addition to this labeling a defined timeslice of a protein (pulse-labeling) and sequential dual color labeling (pulse-chase) opens new opportunities. At the SBS meeting, Covalys will introduce a sibling of the SNAP-tag – the CLIP-tag – to enable orthogonal dual color labeling of two different proteins. Results discussed will include studies on protein dynamics in differentiating myocytes, nuclear protein dynamics during cell division, cell wall growth in budding yeast, and the dynamics of the actin cytoskeleton. Further results will reference studies on GPCR dynamics and nuclear hormone receptor dynamics. The extension of the number of orthogonal self-labeling protein tags will further add to the flexibility of highly specific covalent protein labeling techniques.