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P2044
Application of plex-in-plex Branched DNA Assay in Quantitating mRNA in Early Drug Discovery
Presenter Pramod Pandey, Novartis Institute for Biomedical Research, USA
Additional Authors: Lisa Dzurenda, Thao Duong, Wendy Broom, Nina Nguyen, Son Bui, Jessie Wu, Quan Nguyen, and W. Adam G. Hill
Changes in mRNA levels can be a sensitive indicator of perturbations within a cell. In many diseases there are changes in mRNA levels and a number of drug discovery efforts have been focused on modulating the changes in protein levels or activities that these changes in message evoke. Monitoring mRNA levels in these disease states has several advantages over biochemical assays or reporter gene assays, including  a more proximal readout to the perturbation; the generic nature of the readout, which could be considered label free; and the use of native cells. We present here a relatively straight forward, automatable, multiplex and cost effective approach to mRNA quantitation and its application in medium and high-throughput screening.