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P2048
Application of EpicÒ System in Cell-based Assays
Presenter Hong Xin, JNJ PRD
Additional Authors: Hong Xin, Wang, Yuanping, Lisa Minor and Matthew Todd
The EpicÒ system is a high-throughput, label-free assay system that uses optical waveguide technology to detect changes in local refraction index. These changes correspond to binding event for biochemical assays, and mass redistribution in cell-based assays. The system allows us to assay GPCRs using native cell lines with an endogenous level of receptor expression, regardless of the G-protein coupling mechanism. Because the same assay platform may be used for all GPCR targets, we anticipate a rapid assay development time, the elimination of label-dependent interference, and results that are more predictive of a compound’s physiological activity. This system also enables screening of challenging targets, such as Gi-coupled GPCRs. This presentation would discuss the application of EpicÒ system in functional cell-based GPCR assays for lead generation, and compound profiling.