Sponsored by:
P2061Development of Customized Immunoassays using AlphaLISA®
Presenter Sara Howland, PerkinElmer Life & Analytical Sciences, Canada
Additional Authors: Chantal Illy, C., Bédard, J., Boissonneault, M., Caron, M., Fafard, C., Pinard, G., and Dahan, S.
Competitive and non-competitive (sandwich) immunoassays, such as ELISA assays, are widely employed in laboratories to measure the concentration of a substance in biofluids, typically serum, plasma, urine, cell lysates and cell culture supernatants. These assays take advantage of the specific binding of an antibody to its antigen. The recently introduced AlphaLISA technology provides the advantage of specificity of an immunoassay without the disadvantages associated with wash assays (tedious assays with long incubation times and low throughput densities). The high versatility and sensitivity features make the AlphaLISA platform an excellent choice for the rapid conversion of any ELISA tests to cost-effective miniaturized homogeneous assays.
This poster presents key examples of AlphaLISA assays that were developed for the detection of various analytes from the measurement of biomarkers in serum/plasma samples, to the detection of phosphorylated targets in cell lysates, analysis of secreted proteins from cell culture supernatants, as well as host cell impurity testing of therapeutic antibody solutions. AlphaLISA has been demonstrated to be a remarkable analytical method combining multiple benefits and allowing for quick and easy implementation, especially in high-throughput screening laboratories. PerkinElmer offers its expertise in assay development to scientists for the conversion of any immunoassays to AlphaLISA assays.