View All Posters

P2068
PhotoScreen™ luminescent Calcium Assays  Applications on LumiLux® & FLIPRTETRA®
Presenter Giovanna Gualtieri, Axxam SpA, Italy
Additional Authors: Sabrina Corazza, Viviana Agus, Silvia Bovolenta, Lia Scarabottolo, Axxam, Milano, Vincent Dupriez
Ca2+-activated photoproteins are important tools for analyzing all aspects of Ca2+-mediated signal transduction processes in mammalian cells. One of their characteristics is the immediate photon release (flash luminescence) upon Ca2+ binding to the coelenterazine-photoprotein complex, which makes this system extremely useful for studying rapid receptor-ligand interactions or fast acting ion channels involving Ca2+ mobilization.PhotoScreen cell lines express the Photina®  protein, an extremely bright photoprotein generated at Axxam S.p.A and optimized for HTS campaigns. PhotoScreen assays allows the detection of rapid increases in intracellular Ca2+ concentration with almost no background activity and a consequent high signal-to-background ratio and broad range of detection sensitivity. Data generated on the LumiLux® Cellular Screening Platform and FLIPRTETRA system with PhotoScreen cell lines overexpressing different classes of targets will be presented.