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P2070
Introducing the CellKey™384 System, A Label-Free High-Throughput Cell-Based Assay for Functional Screening of Cellular Receptors
Presenter John Proctor, MDS Analytical Technologies, USA
Additional Authors: John Proctor, Debra Gallant, Ryan McGuinness, Yen-Wen Chen, Roger Tang, Ed Verdonk, Trisha Tutana, Qin Chen, Gordon Leung, Julia Michelotti, Anna Aherne
The CellKey™ System is a proven technology for highly informative, robust and easy to perform secondary screening cellular assays in 96-well format.  Here we introduce the CellKey™384 System, a 384-well label-free cellular assay platform for biorelevant drug discovery applications.  Developed with high-throughput labs in mind, this impedance based system features onboard pipettor, tip wash and thermal control in a design amenable to laboratory automation. The label-free, noninvasive and universal nature of the technology enables the analysis of endogenous and transfected receptors in live cells.  In this poster we demonstrate the ease of scale up from 96-well to 384-well assay format without compromising the quality of output data.  Testing target receptors on several cell lines, including suspension cells, we effectively transferred assay workflows, assay protocols and data analysis parameters to the high-throughput system.  Consistency of data in terms of CellKey™ response profiles, Z’ and EC50/ IC50 values are demonstrated across both platforms over multiple days.  The data featured here show that the CellKey™384 System enables simple and fast investigation of targets in a high-throughput biorelevant drug discovery paradigm.