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P2083
HTS Flow Cytometry of Multiplexed Targets HTS Flow Cytometry
Presenter Larry Sklar, University of New Mexico, USA
Additional Authors: W. Lehmann, R. Hiemann, K. Grossmann, L. Pfeifer
It has become practical to use the multi-parameter optical capabilities of the flow cytometer for the discovery of active small molecules from compound libraries by HTS. We have improved the efficiency of HTS by increasing biological content through analysis of multiplexed targets. Multiplexing is achieved with mixtures of targets that are color-coded as suspension arrays. Several receptors or transporters on distinct cell populations, or several molecular assemblies on distinct microsphere populations are mixed in a single assay well. This approach has been successfully applied to GPCR receptor families, MDR transporters, low molecular weight G proteins, Bcl-2 family interactions, and substrate families for proteases. The data (more than 1 million data points to date) reveal small molecules with selectivity and specificity in both HTS primary screens and dose-response analysis. Session Advances in Automation & Detection Platforms