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Sponsored by:
P3017
Advances in High-Throughput Screening with the New LumiLux® CS Cellular luminescence Platform.
Presenter Lee Brady, PerkinElmer
Additional Authors: Craig Malcolm, David Bourdon, Gary Reznik, Brian Gallagher, Janet Park, Timothy Smith, Mitchell Hull, Deborah Nguyen, and Daniel Sipes
Drug discovery and development requires the use of highly sensitive cell-based assays. PerkinElmer delivers a complete solution for such demands. That solution includes all the components to make the cell screening a simple turnkey process for any lab  reliable flexible liquid handling, cell delivery, highly sensitive detection based on ‘contact imaging’ (LumiLux® and LumiLux CS) combined with PerkinElmer’s proprietary recombinant Aequorin and Photina® photoprotein expressing cell lines that can be used to measure changes in intracellular calcium.Both the LumiLux Cellular Screening Platform and the new LumiLux CS Cellular Luminescence Platform offer the advantage of true uHTS/HTS automated cellular assay screening that result in a decrease in labor and material costs, with increased throughput and productivity. LumiLux capabilities include liquid-handling/automation; user-friendly WinPREP® software; independent cell-suspension capabilities; ultra-sensitive luminescence detection, and complete data-analysis solutions using AssayPro). The fully enclosed LumiLux provides a means for truly independent (unattended) overnight assays at high-throughput/density volumes.
For labs seeking an interface with “existing” automation, LumiLux CS provides the optimal solution. With reduced footprint, the LumiLux CS provides an external “shuttle-feed” designed for interfacing with existing robotics and incubator systems. Both LumiLux and LumiLux CS use 384 and 1536 well formats with corresponding pipetting heads.The focus of this poster is to present comparative performance data for the LumiLux with LumiLux CS. The data will include comparisons of 384 and 1536 plate assay data throughput capacities, footprint and space requirements, and overall detection sensitivity.