Sponsored by:
P5002Automation of AlphaLISA Insulin & VEGF Immunoassays on JANUS Liquid Handling Workstation
Presenter Dianne Brazzill, PerkinElmer, USA
Additional Authors: Claire Normand, Stephane Parent, Veronique Brechler, and Gary Reznik
Vascular Endothelial Growth Factor (VEGF) is a key regulator of angiogenesis and vasculogenesis in a variety of physiological and pathophysiological processes. In pathological conditions, this growth factor has been shown to be involved in tumor growth and vascularization. As such, it is a molecule of great interest in basic research, drug discovery, and clinical research. Insulin, too, is studied extensively due to its role in diabetes and its management, metabolic syndrome, and as a biomarker for other conditions.
The increasing need for VEGF and insulin quantitative determinations have been successfully addressed by automating VEGF and insulin AlphaLISA® assays on the JANUS® AlphaLISA Workstation. The data presented in this poster demonstrate accuracy, precision, recovery, and throughput capabilities of the JANUS AlphaLISA workstation when it is used for VEGF and insulin quantitative determinations. Our results demonstrate a very high level of accuracy and precsion can be achieved, even for very small reagent volumes (5 microliters). Combined with the ability to process samples in 96-, 384-, and 1536-well plate formats, JANUS AlphaLISA workstation presents a strong alternative to the classic VEGF and insulin ELISA methods.