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P6001
Validation of Low Volume 1536 “Assay Ready” Compound Plates for a Nuclear Hormone Receptor Screen.
Presenter Bruno Becker, AstraZeneca, Sweden
Additional Authors: Hans Eriksson, Johan Brengdahl, Anders Hogner
The predispensing of compound libraries by acoustic droplet ejection is a convenient way of preparing ”assay ready” compound plates. This method also allows for the direct (i.e. no predilution necessary) and precise dispensing of the low volumes of compound DMSO solution required for assays in 1536 plates. However, questions arise about the effects of time, temperature and dispensed volume on assay performance and repeatability. These issues about the “shelf-life” of assay ready plates are of particular importance if large batches of plates need to be prepared several days in advance, e.g. for HTS screens. In an effort to validate this method of compound plate preparation for a particular screen and compound set, we predispensed agonists of a nuclear hormone receptor in 25, 75 and 150 nl volumes of DMSO in triplicate 1536 plates at different time points prior to performing a TR-FRET agonist assay (plates prepared 2 weeks, 1 week, 2 days, 1 day, 0 days before and stored sealed at room temperature). In addition, we kept a 2 week old plate set at –18 oC to see if storage temperature had an effect on compound activities. We will present a comparison and interpretation of the assay EC50 results between these plate sets.