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P8008
A Heterogeneous Angiogenesis Assay Using Primary Cell Co-Culture In Microchannels
Presenter Allyson Skoien, BellBrook Labs, USA
Additional Authors: Victoria Echeverria, Tracy Worzella, Allyson Skoien, Casey Lamers, Ivar Meyvantsson, Steven Hayes
When primary endothelial cells are co-cultured with primary diploid fibroblasts, structures resembling a microvasculature bed can be obtained. No exogenous extracellular matrix is required, and standard endothelial growth media containing low (2%) serum is sufficient. This previously-developed cell model has been adapted for highly parallel operation in an array of microchannels. The microchannel arrays are designed to allow fully automated operation, including media changes and immunocytochemistry wash steps, all using standard liquid handling automation. Presented are results of validation using test compounds.