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P9009
Automated Low Volume Cell Dispensing & Analysis Using the BioRAPTR FRD Microfluidic Workstation & PARADIGM™ Detection Platform for cAMP & Ion Channel Assays
Presenter Li Liu
Additional Authors: Laura Pajak
The protocols described in this poster were used to generate low-volume cell culture plates, to add agonists for cell stimulation and to dispense reagents for the cell-based assays.   Two assays were selected to demonstrate automation in 384- and 1536-well plates.  A GPCR cell-based assay, the cAMP dynamic 2 kit from Cisbio, measures cyclic AMP levels using HTRF* technology and was automated in a 1536-well format.  Because ion channel activity is increasingly being tested in screening laboratories, the Fluo-4 NW Calcium Assay Kit from Invitrogen was used to measure the cytosolic Ca2+ signal upon stimulation and was automated in a 384-well format.  The detection and analysis were performed using the PARADIGM Detection Platform from Beckman Coulter.The data in this poster show the accuracy of cellular dispensing and the results obtained from the two screening assays as automated on the BioRAPTR FRD workstation. @BioRAPTR FRD For research use only; not for use in diagnostic procedures. *All trademarks are property of their respective owners.